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KMID : 0379520080240010059
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한국독성학회지
2008 Volume.24 No. 1 p.59 ~ p.68
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Studies on N-Ethyl-N-nitrosourea Mutagenesis in BALB/c Mice
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Cho Kyu-Hyuk
Cho Jae-Woo
Song Chang-Woo
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Abstract
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N-ethyl-N-nitrosoures (ENU) is effective in inducing hypermorphic mutation as well as hypomorphic and antimorphic mutations. Therefore, this mutagen is used to the production of mutant in the mice. In order to perform an effective ENU mutagenesis using BALB/cAnN mice, determination of optimal dosage and dosage regimen of ENU is necessary. And this study tried to develop a suitable screening method and searched for novel and various mutants as model animals in phenotypedriven ENU mutagenesis. We have carried out dosage regimen for mutagenizing dose of 200 ㎎/㎏ ENU in the BALB/c mice. Total screened mice were 30,133. As the results of Esaki and Cho’s Phenotype Screening, we got 2,516 phenotypic and behavior abnormalities in G₁, G₂and G₃mice. One hundred thirty five G₁phenodeviants were tested for inheritance and 16 dominant mutants were discovered. Forty two recessive mutants were also found in tested 201 micropedigrees. Early-onset mutant mice included the dysmorphology of face, eye, tail, limb, skin, and foot and abnormal behavior like circling, swimming, head tossing, stiff-walking, high cholesterol level, and tremor etc. In this study we could effectively screen G₃recessive mutants. The frequent and concise early-onset screening before weaning will be available for ENU mutagenesis.
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KEYWORD
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ENU mutagenesis, Mutant, Dose, ECPS, BLAB/cAnN mice
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